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. 2004 Dec 15;18(24):3016–3027. doi: 10.1101/gad.1262504

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Figure 6. — Homodimerization of Drosha and DGCR8. (A) Flag-tagged and V5-tagged Drosha proteins were coexpressed in HEK293T cells by transient transfection of the indicated plasmids. Forty-eight hours post-transfection, total cell extract was prepared and used for immunoprecipitation with anti-Flag affinity gel. Proteins were analyzed by Western blotting with anti-V5 antibody (upper panel) or anti-Flag antibody (lower panel). V5-Dicer was used as a control. (B) Flag-tagged and V5-tagged DGCR8 proteins were coprecipitated. Immunoprecipitation and Western blot analysis were done as in A.