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. 2004 Dec 15;18(24):3117–3130. doi: 10.1101/gad.314904

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Copyright © 2004, Cold Spring Harbor Laboratory Press

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Figure 6. — The MAPK consensus sites of Crk1 are required for in vivo activity. (A) Scheme of crk1 as well as crk1^AAA alelles. The MAPK consensus sequences are shown. (B) The MAPK consensus sites are dispensable for mating. FB1, FB2, UMP12 (FB1 Δcrk1), UMP14 (FB2 Δcrk1), UMS13 (FB1 crk1^AAA), and UMS15 (FB2 crk1^AAA) cells were cospotted in charcoal-containing PD plates. (C) The MAPK consensus sites are required for filamentation induced by defects in cAMP pathway. FB1, SONU24 (FB1 Δadr1), UMS13 (FB1 crk1^AAA), and UMS14 (FB1 Δadr1 crk1^AAA) were incubated in liquid YPD medium until exponential growth. Bar, 20 μm. (D) The MAPK sites are required for Crk1-mediated hyperpolarized growth. UMP12 (FB1 Δcrk1), UME61 (FB1 Δcrk1 P_crg:crk1-myc), and UMS12 (FB1 Δcrk1 P_crg:crk1^AAA-myc) cells were incubated in CMD and CMA for 6 h. Bar, 10 μm.