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. 2004 Dec 15;18(24):3117–3130. doi: 10.1101/gad.314904

Figure 8.

Figure 8.

Crk1 is required for the induction of the sexual program. (A) Constitutive expression of b locus bypassed the requirement of Crk1 for infective filament formation. The strains indicated at top were spotted on charcoal-containing PD plates. HA103 carries b-compatible alleles expressed under a constitutive promoter. (B) Constitutive expression of the pheromone receptor gene partially suppresses the mating defect of Δcrk1 cells. The strains indicated at top, left were cospotted on charcoal-containing PD plates. (C) Crk1 is required for the trigging of pheromone expression after pheromone stimulation. The strains indicated at top were treated for 5 h with synthetic a2 pheromone dissolved in DMSO (+) or with the same volume of DMSO (-). RNA was isolated, and 10 μg of total RNA was loaded per lane. The blot was probed with mfa1 and with rRNA as loading control. (D) Requirement of Crk1 in pheromone induction is located downstream of Fuz7. The strains indicated at top were grown with glucose (-) or with arabinose (+) as a carbon source. RNA was isolated, and 10 μg of total RNA was loaded per lane. The blot was probed with mfa1 and with rRNA as loading control.