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. 2017 Mar 21;3:17006. doi: 10.1038/celldisc.2017.6

Figure 6.

Figure 6

ZIKV nonstructural proteins co-operate to evade IFN restriction. (a) Quantitative PCR with reverse transcription (qRT-PCR) analysis of IFNβ mRNA in 293T cells transfected with various combinations of plasmid for NS1, NS2B3 and NS4B followed by SeV (MOI=0.1) infection for 9 h. (b) qRT-PCR analysis of IFIT1, IFIT2, MX1, ISG15 and RIG-I mRNA in 293T cells transfected with various combinations of plasmid for NS1, NS2B3 and NS4B followed by SeV infection (MOI=0.1) for 9 h. (c) qRT-PCR analysis of SeV phosphoprotein RNA (pRNA) in 293T cells transfected with various combinations of plasmid for NS1, NS2B3 and NS4B followed by SeV infection (MOI=0.1) for 9 h. (d) Plaque titration of VSV in supernatant of 293T cells transfected with various combinations of plasmid for NS1, NS2B3 and NS4B followed by VSV infection (MOI=0.01) for 24 h. (e) Immunoassay of extracts of 293T cells transfected with HA-NS2B3, together with Flag-NS1 or NS4B, then infected with or without SeV (MOI=0.1). Data in a, b and c are expressed as means±s.d. of at least three independent experiments. *P<0.05, **P<0.01, ***P<0.001. NS, not significant.