Table 1.
The primer sequences used for real-time PCR assay in the present work.
| Gene | GenBank accession | Forward primer (5’–3’) | Reverse primer (5’–3’) |
|---|---|---|---|
| Mouse GAPDH | NM_008084.2 | TGTGTCCGTCGTGGATCTGA | TTGCTGTTGAAGTCGCAGGAG |
| Mouse TNF-α | NM_013693.2 | TATGGCCCAGACCCTCACA | GGAGTAGACAAGGTACAACCCATC |
| Mouse IL-1β | NM_008361.3 | TCCAGGATGAGGACATGAGCAC | GAACGTCACACACCAGCAGGTTA |
| Mouse IL-6 | NM_031168.1 | CCACTTCACAAGTCGGAGGCTTA | CCAGTTTGGTAGCATCCATCATTTC |
| Rat GAPDH | NM_017008.3 | GGCACAGTCAAGGCTGAGAATG | ATGGTGGTGAAGACGCCAGTA |
| Mouse IL-1β | NM_031512.2 | CCCTGAACTCAACTGTGAAATAGCA | CCCAAGTCAAGGGCTTGGAA |
| Rat IL-6 | NM_012589.1 | ATTGTATGAACAGCGATGATGCAC | CCAGGTAGAAACGGAACTCCAGA |
| Rat TNF-α | NM_012675.3 | TCAGTTCCATGGCCCAGAC | GTTGTCTTTGAGATCCATGCCATT |
| Human GAPDH | NM_002046.3 | GCACCGTCAAGGCTGAGAAC | TGGTGAAGACGCCAGTGGA |
| Mouse IL-1β | NM_000576.2 | CTGAGCACCTTCTTTCCCTTCA | TGGACCAGACATCACCAAGCT |
| Human IL-6 | NM_000600.3 | TGGCTGAAAAAGATGGATGCT | TCTGCACAGCTCTGGCTTGT |
| Human TNF-α | NM_000594.3 | TGTAGCCCATGTTGTAGCAAACC | GAGGACCTGGGAGTAGATGAGGTA |