Table 2.

Comparative assessment of isomiR profiling using isomiR2Function and isomiRID.

	isomiR2Function						isomiRID
	Templated			Non-templated			Templated			Non-templated
	ath	bdi	osa	ath	bdi	osa	ath	bdi	osa	ath	bdi	osa
Total	383	1613	2734	3959	8881	10946	383	1613	2734	3905	6869	10946
Complex	120	230	541	2299	2471	3836	120	230	541	2284	2270	3836
Canonical	200	629	836	1504	3748	2921	200	629	836	1499	3108	2921
Non-canonica	63	754	1357	156	2662	4189	63	754	1357	122	1491	4189
5a	14	28	33	250	133	82	14	28	33	250	111	82
3a	41	42	61	392	491	299	41	42	61	391	474	299
5d	17	53	89	29	440	189	17	53	89	29	411	189
3d	47	98	132	149	553	323	47	98	132	148	520	323
5a3a	6	9	18	69	39	30	6	9	18	67	22	30
5a3d	50	164	219	212	461	442	50	164	219	212	242	442
5d3a	17	210	224	44	511	549	17	210	224	44	289	549
5d3d	8	25	60	8	97	67	8	25	60	7	62	67
No drift	0	0	0	351	1023	940	0	0	0	351	977	940
Both	383	1613	2734	3905	6869	7877	383	1613	2734	3905	6869	7877
Specific	0	0	0	54	2012	3069	0	0	0	0	0	0

^∗ath = Arabidopsis thaliana; bdi = Brachypodium distachyon; osa = Oryza sativa; Complex, isomiRs with multiple origin; Canonical, isomiRs have enough bases overlapped with canonical miRNAs; Non-canonical, isomiRs do not have enough bases overlapped with canonical miRNAs; 5a = 5′ terminal addition; 3a = 3′ terminal addition; 5d = 5′ terminal deletion; 3d = 3′ terminal deletion; 5a3a = 5′ terminal addition and 3′ terminal addition; 5a3d = 5′ terminal addition and 3′ terminal deletion; 5d3a = 5′ terminal deletion and 3′ terminal addition; 5d3d = 5′ terminal deletion and 3′ terminal deletion; No drift, isomiRs identified at the same place of canonical miRNAs on the pre-miRNAs; Both, identified by both tools; Specific: Identified specifically. isomiRID available from https://github.com/lfelipedeoliveira/isomiRID