Fig. 2.

Tomographic slices and three-dimensional models of a control cell Golgi apparatus stack in a and b, and a Golgi body of a cell treated with 2DG for 45 min in c are shown. In both cases, the cell cultures were high-pressure frozen, freeze substituted and embedded in Epon. In contrast to the parallel organization of the cisternae that build up a Golgi stack in the control cell (b), the 2DG-treated cell shows various, in part tubular, cisternal and small vesicular compartments, that form a loosely arranged Golgi body (c). Branched and bifurcated structures (white arrows) are common. Volumes of the calculated tomograms (x, y, z): a, b 3.535 × 2.919 × 205 pixels, pixel size 0,59 nm; c 4.073 × 3.943 × 140 pixels, pixel size 0,46 nm. d The percentage ATP-values after 45 min and 5 h of treatment of HepG2 cell cultures with 2DG of different, 10, 25 and 50 mM, concentrations. The graphic shows that a massive reduction of the ATP-levels is obtained with each of the 2DG-concentrations tested. The data shown are taken from one representative experiment; comparable results were obtained in repeated experiments