Fig. 3.

a, b Percentage ATP-values are shown as obtained after different times of culture in either glucose-pyruvate-free medium (GPF) containing 50 mM 2DG or in GPF lacking 2DG, respectively. The curve of declining ATP-values in a indicates that the main ATP-reduction occurs within the first 10 min of treatment. By contrast to the considerably reduced ATP-levels measured in the 2DG-treated cell cultures, the results obtained with the cultures grown in GPF without 2DG show ATP-values comparable to the controls or slightly reduced at the later times of treatment. The data are taken from one representative experiment; repeated experiments yielded comparable results. c, d The Golgi apparatus morphologies of cells cultured for 10, 30 and 180 min in GPF with or without 2DG, respectively; the respective ATP-values are additionally indicated in the right upper corner of the pictures. c Alterations of the Golgi apparatus stack are hardly visible after 10 min of 2DG-treatment; the stack appears disorganized and in part changed in a loosely arranged Golgi body after 30 min; a Golgi body composed of densely packed membrane compartments is seen after 180 min of treatment. d By contrast, cells cultured in GPF without 2DG show unchanged Golgi apparatus stacks comparable to those of control cells. In all pictures, RER-cisternae are found nearby the Golgi stacks exhibiting denser contents in the 2DG-treated cells (c) than in those cultured without 2DG