Fig. 6.

Tomographic slices in a–d obtained from different levels of a Golgi apparatus reconstruction at 15 min of 2DG-treatment show that different sites within a stack are interlinked by crossroads-like junctions, accompanied by frequent bifurcations. A representative area is on display in a, b and d and is further accentuated by means of drawings and by numbering some of the cisternae (c, e–g). Two different sites within the stack are labeled in red and blue and their spatial relationship traced within the reconstructed volume. The colored regions are found apart from each other in slice 200 (a, e); in slices 131 and 142, they can be seen joined forming parts of a crossroads-like junction (b, c, f), and they are separated again in slice 80 (d, g). The numbers in the left lower corner of a-d indicate the respective numbers of the slices. Volume of the calculated tomogram (x, y, z): 3.585 × 3.604 × 242 pixels, pixel size 0.39 nm