Table 1.

Primer sequences for PCR amplification of ELA2 exon 2–5

ELA2 target sequence	Primers
Exon 2 F	5'-tgtaaaacgacggccagtgggaggggacaggctccttgg-3'
Exon 2 R	5'-caggaaacagctatgaccaccgggacgcggggtccgagc-3'
Exon 3 F	5'-tgtaaaacgacggccagtcaggcccgtcgccggatggg-3'
Exon 3 R	5'-caggaaacagctatgacctccgtcgcagcctccaccct-3'
Exon 4 F	5'-tgtaaaacgacggccagtgtgacgcgctgacgatctgt-3'
Exon 4 R	5'-caggaaacagctatgaccgcagtaccgggctgggagcg-3'
Exon 5 F	5'-tgtaaaacgacggccagtcagtccagcttccccacctt-3',
Exon 5 R	5'-caggaaacagctatgaccgacctactgaccattttcaac-3'

PCR primers sequences for ELA2 exon 2–5, for following sequencing reactions with BigDye primer, Applied Biosystems.