FIGURE 2.

Autoradiograms to determine specific bindings of tritium-labeled geissoschizine methyl ether ([³H]GM) and 18β-glycyrrhetinic acid ([³H]GA) in rat brain slices. Freshly frozen sections of rat brain were reacted with [³H]GM or [³H]GA and exposed to a tritium-sensitive imaging plate. After exposure, the plates were analyzed in an imaging analyzer to generate autoradiograms. Specific binding sites of [³H]GM were densely detected in the frontal cortex and those of [³H]GA were in the hippocampus. These findings are cited from the following articles: Mizoguchi et al. (2014a) for [³H]GM; Mizoguchi et al. (2014b) for [³H]GA.