TABLE 1.
S. cerevisiae proteins containing at least two Fpk1 phospho-acceptor site motifs
| Protein/ORF | Function | Fpk1 motifs | References describing evidence for phosphorylation in vivo |
|---|---|---|---|
| Dnf1/YER166W | Aminoglycerophospholipid translocase (flippase) | RSS348LD, RVS358AD, RPS365LD, RSS1526LD, RYS1545VE, RTS1552LD | 23, 68, 98–102 |
| Dnf2/YDR093W | Aminoglycerophospholipid translocase (flippase) | RGS386LD, RMS396AD, RPS403LD, RTS1566LD, RAS1592LD | 23, 32, 68, 98–104 |
| Dnf3/YMR162C | Aminoglycerophospholipid translocase (flippase) | RPS651LD, RNS661IE, RKS88LE, RIS973ID | 68, 102, 105–107 |
| Ypk1/YKL126W | Ser/Thr protein kinase controlling PM lipid and protein homeostasis | RSS51LD, RVS71YD | 23, 64, 102 |
| Akl1/YBR059C | Ser/Thr protein kinase; member, with Ark1 and Prk1, of a protein kinase family involved in control of endocytosis and actin cytoskeleton organization | RQS960LD, RQS1072LD | 32, 68, 98, 100–104; this study |
| Cti6/YPL181W | PHD domain-containing component of the Rpd3L histone deacetylase complex | RNS216MD, RRS406AD | 32, 68, 98, 99, 101, 103, 104 |
| Dit2/YDR402C | N-Formyltyrosine oxidase | RES62ME, RWS459LD | |
| Ent4/YLL038C | Protein of unknown function (contains an N-terminal epsin-like domain) | RQS182LE, RFS213LD | 68, 101, 102, 104 |
| Erg1/YGR175C | Squalene monooxygenase | RPS302FD, RKS386ID | |
| Ira2/YOL081W | GTPase-activating protein for Ras1 and Ras2 (paralog of Ira1) | RAS547YD, RLS779ID | |
| Irc20/YLR247C | E3 ubiquitin ligase and putative helicase | RKS152LE, RFS224VE, RES298VE | |
| Lcb5/YLR260w | Long-chain (sphingoid) base kinase (paralog of Lcb4) | RSS55ID, RCS302IE | 68, 98, 102, 104 |
| Mks1/YNL076W | Negative transcriptional regulator (with pleiotropic roles in lysine biosynthetic pathway and nitrogen regulation and in both Ras-cAMP and retrograde [RTG] mitochondrion-to-nucleus signaling) | RLS453MD, RQS518MD | 32, 68, 100–102, 104 |
| Not3/YIL038C | Subunit of CCR4-NOT global transcriptional regulator | RRS83VE, RSS348AD | |
| Pfa3/YNL326C | Palmitoyl coenzyme A transferase of the DHHC-CRD family required for C-terminal S-palmitoylation of vacuolar membrane protein Vac8 | RPS322LE, RAS329VE | 68, 102 |
| Pkh3/YDR466W | Protein kinase (weak similarity to Pkh1 and Pkh2 and mammalian PDK1) | RIS283LE, RNS499ID | |
| Prp2/YNR011C | RNA-dependent DEXD/H-box ATPase required for spliceosome activation | RAS545VD, RKS619LE | |
| Tus1/YLR425W | Guanine nucleotide exchange factor for Rho1 | RKS27IE, RPS1163IE | 101, 102, 106, 107 |
| Vps54/YDR027C | Component of the Golgi-associated retrograde protein complex | RLS69LD, RRS78FD | 68, 98, 99, 102–104, 108 |
| Yta6/YPL074W | Cortically localized putative AAA+ ATPase of the Cdc48/Pas1/Sec18 subfamily | RAS255LD, RRS278LD, RKS296ME | 32, 68, 98–101, 104 |