TABLE 2.
S. cerevisiae strains used in this study
| Strain | Genotype | Source or reference |
|---|---|---|
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Research Genetics, Inc. |
| YFR205 | BY4741 fpk1Δ::KanMX4 fpk2Δ::KanMX4 lys2Δ0 | 23 |
| YJW2 | BY4741 Fpk111A::HIS3 | 31 |
| YFR437 | BY4741 Akl1-mCherry::CaURA3 | This study |
| YFR468 | BY4741 Fpk111A::HIS3 Akl1-mCherry::CaURA3 | This study |
| YFR469 | BY4741 fpk1Δ::KanMX4 fpk2Δ::KanMX4 Akl1-mCherry::CaURA3 | This study |
| YFR515 | BY4741 Akl1-mCherry::CaURA3 Sla1-GFP::HIS3 | This study |
| PFY3272C | BY4741 dnf1Δ::KanMX4 dnf2Δ::KanMX4 dnf3Δ::KanMX4 | 109 |
| ypk1Δ mutant | BY4741 ypk1Δ::KanMX4 | Research Genetics, Inc. |
| yKL28 | BY4741 slm1Δ::KanMX slm2Δ::KanMX [pRS416-Ypk1(D242A)-3HA] | This study |
| GFY1770 | BY4741 DNF1::HygMX dnf2Δ::KanMX dnf3Δ::KanMX | This study |
| GFY1773 | BY4741 DNF1-GFP::HygMX dnf2Δ::KanMX dnf3Δ::KanMX | This study |
| GFY1772 | BY4741 DNF1(S348A S358A S365A S1526A S1545A S1552A)::HygMX dnf2Δ::KanMX dnf3Δ::KanMX | This study |
| GFY1775 | BY4741 DNF1(S348A S358A S365A S1526A S1545A S1552A)-GFP::HygMX dnf2Δ::KanMX dnf3Δ::KanMX | This study |
| GFY1728 | BY4741 dnf1Δ::natNT2 dnf2Δ::KanMX dnf3Δ::KanMX | This study |
| BY4742 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | Research Genetics, Inc. |
| ypk2Δ mutant | BY4742 ypk2Δ::KanMX4 | Research Genetics, Inc. |
| YFR381 | BY4742 slm1Δ::KanMX4 slm2Δ::KanMX4 [pRS315-Ypk1(D242A)-Myc] | This study |
| JTY6532 | BY4742 akl1Δ::KanMX | Research Genetics, Inc. |
| YFR474-A | BY4742 Akl1-3×FLAG::URA3 | This study |
| YFR475-A | BY4742 Akl1(S960A S1072A)-3×FLAG::URA3 | This study |
| YFR476-A | BY4742 Akl1(S960E S1072E)-3×FLAG::URA3 | This study |
| YFR479 | BY4742 akl1Δ::Hygr | This study |
| avo3ΔCT TOR1-1 mutant | MATa avo3Δ1274-1430 TOR1-1 trp1 his3 ura3 leu2 rme1 (TB50 strain background) | 38 |
| YFR491-A | MATa ENT2-GFP::HIS3 his3 leu2 ura3 met15 | This study |
| YFR492-A | MATa ENT2-GFP::HIS3 fpk1Δ::KanMX4 fpk2Δ::KanMX4 his3 leu2 ura3 lys2 | This study |
| JTY5180 | MATα ABP1-RFP::HIS3 his3-Δ200 ura3-52 leu2-3,112 (DDY3058) | 110 |
| YFR507 | MATa Akl1::URA3 Sla1-GFP::CgHIS Abp1-RFP::HIS3 his3-Δ200 ura3-52 leu2-3,112 lys2-801 | This study |
| YFR508 | MATa Akl1(S960A S1072A)::URA3 Sla1-GFP::CgHIS Abp1-RFP::HIS3 his3-Δ200 ura3-52 leu2-3,112 lys2-801 | This study |