TABLE 3.
Plasmids used in this study
| Plasmid | Description | Source or reference |
|---|---|---|
| pGEX4T-1 | GST tag, bacterial expression vector | GE Healthcare, Inc. |
| pAX15 | pGEX4T-1 Fpk1-GSGSHHHHHH | This study |
| pJY10 | pGEX4T-1 Fpk1(D621A)-GSGSHHHHHH | This study |
| pFR290 | pGEX4T-1 Akl1(767-1108) | This study |
| pFR293 | pGEX4T-1 Akl1(767-1108; S960A) | This study |
| pFR294 | pGEX4T-1 Akl1(767-1108; S1072A) | This study |
| pFR297 | pGEX4T-1 Akl1(767-1108; S960A S1072A) | This study |
| pGEX3 | GST tag, bacterial expression vector | GE Healthcare, Inc. |
| pDD0214 | pGEX3 Sla1(854-918) | Drubin lab, UC Berkeley |
| pTS408 | CEN URA3 GAL1prom GFP vector | 111 |
| pDD0938 | pTS408 GFP-Akl1 | Drubin lab, UC Berkeley |
| pFR303 | pTS408 GFP-Akl1(S960A S1072A) | This study |
| pKL31 | pTS408 GFP-Akl1(D181A S960A S1072A) | This study |
| pFR304 | pTS408 GFP-Akl1(Δ30-751) | This study |
| pFR329 | pTS408 GFP-Akl1(Δ30-751; S960A) | This study |
| pFR328 | pTS408 GFP-Akl1(Δ30-751; S1072A) | This study |
| pFR334 | pTS408 GFP-Akl1(Δ30-751; S960A S1072A) | This study |
| pRS315 | CEN LEU2 | 112 |
| pFR234 | pRS315 Ypk1(D242A)-Myc | This study |
| pFR316 | pRS315 Akl1-3×FLAG | This study |
| pFR318 | pRS315 Akl1(D181Y)-3×FLAG | This study |
| pFR319 | pRS315 Akl1(S960A S1072A)-3×FLAG | This study |
| YCpLG | CEN LEU2 GAL1prom vector | 113 |
| pFR360 | YCpLG 3×FLAG-Sla1(851-1244) | This study |
| pRS416 | CEN URA3 vector | 112 |
| pPL215 | pRS416 MET25prom Ypk1-3×HA | 9 |
| pKL27 | pRS416 MET25prom Ypk1(D242A)-3×HA | This study |