TABLE 1.
Oligonucleotides used for real-time PCR
| Gene | Accession no. | Sequence (5′–3′) |
Size (bp) | |
|---|---|---|---|---|
| Forward | Reverse | |||
| Rptor | NM_028898.2 | TGGGTCTTCAACAAGAACTACACT | TCTGGGCAAGTGGATGGTTT | 199 |
| Runx2 | NM_001146038.2 | ATGATGACACTGCCACCTCTG | ATGAAATGCTTGGGAACTGC | 120 |
| Sp7 | NM_130458.3 | ATGGCGTCCTCTCTGCTTG | GTCCATTGGTGCTTGAGAAGG | 211 |
| Bglap | NM_007541.3 | AAGCAGGAGGGCAATAAGGT | TCAAGCCATACTGGTCTGATAGC | 142 |
| Spp1 | NM_001204201.1 | AGCAAGAAACTCTTCCAAGCAA | GATTCGTCAGATTCATCCGAGT | 130 |
| Sparc | NM_009242.5 | ACTACATCGGACCATGCAAATAC | CGTACAAGGTGACCAGGACAT | 108 |
| Ibsp | NM_008318.3 | CAGTCCAGGGAGGCAGTG | GGAAAGTGTGGAGTTCTCTG | 158 |
| Col10a1 | NM_009925.4 | CATCTCCCAGCACCAGAATC | GCTAGCAAGTGGGCCCTTTA | 142 |
| Sox9 | NM_011448.4 | CACAAGAAAGACCACCCCGA | GGACCCTGAGATTGCCCAGA | 209 |
| Mmp13 | NM_008607.2 | AGCAGTTCCAAAGGCTACAACT | CCTCGGAGACTGGTAATGGC | 269 |
| Col2a1(II) | NM_001113515.2 | GCGACCGGGAGCATATAACT | GCCCTAATTTTCGGGCATCC | 290 |
| Actb | NM_007393.4 | TTGCTGACAGGATGCAGAAG | AAGGGTGTAAAACGCAGCTC | 236 |
| Alpl | NM_007431.3 | GCCTTACCAACTCTTTTGTGC | GGCTACATTGGTGTTGAGCTT | 116 |
| Gli1 | NM_010296.2 | TACCATGAGCCCTTCTTTAGGA | GCATCATTGAACCCCGAGTAG | 116 |