FIG 1.

Generation of KLHL3 knockout/lacZ knock-in mice. (A) Targeting strategy for generating KLHL3 knockout/lacZ knock-in mice. The diagram shows the wild-type KLHL3 locus, the targeting vector, and the targeted locus before and after Cre recombination. The targeting vector was designed to delete exon 3 of KLHL3. In addition, this vector led to the expression of the lacZ gene under the control of the endogenous KLHL3 promoter. EN2-SA, Engrailed-2 splice acceptor. (B) Verification of homologous recombination and genotyping PCR of the genomic DNA of the mice.