FIG 3.

Tissue and intratissue distribution of KLHL3 was confirmed by an immunoblot of β-Gal and LacZ staining. (A) Immunoblotting to detect the β-Gal protein level in each organ from KLHL3 knockout/lacZ knock-in mice. Representative immunoblots of β-Gal in each of the organs of wild-type and KLHL3 homozygous knockout/lacZ knock-in mice are shown. β-Gal was strongly detected in the kidney and brain and weakly detected in the eye, testis, lung, heart, liver, stomach, and colon. (B) LacZ staining of the kidney and brain in KLHL3 knockout/lacZ knock-in mice. Strong LacZ staining was observed in the hippocampus and cortex of the brain and in the distal convoluted tubules of the kidney. (C) Double immunofluorescence of β-Gal and the Na-Cl cotransporter (NCC) in the kidneys of wild-type and KLHL3 knockout/lacZ knock-in mice. The β-Gal signal colocalized with NCC, indicating that KLHL3 was present in the distal convoluted tubules. Scale bars, 10 μm.