FIG 4.

WNK kinases only increased in the kidneys of KLHL3^−/− mice. (A) Representative immunoblots of β-Gal and WNK1, WNK3, and WNK4 in KLHL3-expressing tissues in KLHL3^+/+ and KLHL3^−/− mice. In KLHL3^−/− mice, the expression levels of WNK1, WNK3, and WNK4 did not increase in any organ other than the kidney, where WNK1 and WNK4 increased. N.D., not detected. (B) Immunoblotting of WNK1 and WNK3 protein levels in hippocampus and cortex from KLHL3^−/− mice. No significant differences in WNK protein levels were detected between wild-type and KLHL3^−/− mice. We repeated the same experiments three times with consistent results.