FIG 3.

Long-term Wolbachia infection in Drosophila S2 cells after coculture with infected JW18 cells in a transwell chamber. Uninfected Drosophila S2 cells were seeded beneath Wolbachia-infected JW18 cells in a transwell insert. After coculture for 3 days, the transwell insert containing infected cells was removed, and new medium was added to the previously uninfected S2 cells. S2 cells were then cultured for an additional 18 days (21 days total), and Wolbachia infections were visualized by FISH and DIC. Red, Wolbachia; blue, nuclei stained with DAPI.