TABLE 2.

Glycoside hydrolases found in extracts of spruce wafer sections degraded by Gloeophyllum trabeum and the number of unique peptides identified for each protein in each wafer section extract

G. trabeum no.a	GH familyb	Putative functionc	SignalP resultsd	No. of unique peptides at distance (mm)e:
				0–5	10–15	20–25
81012	1	β-Glucosidase	No	13	20	15
115191	2	Mannanase	Yes	12	13	11
44548	3	β-Glucosidase	Yes	2	0	0
71534	3	β-Glucosidase	Yes	0	6	0
122002	3	Exo-1,4-β-xylosidase	Yes	8	10	7
75899	3	β-Glucosidase	No	7	8	7
69843	3	β-Glucosidase	Yes	10	13	13
68070	5	Exo-1,3-β-glucanase	Yes	2	0	0
118009	5	Unknown	Yes	2	2	0
120257	5	Endo-1,4-β-glucanase	Yes	2	4	2
41779	5	Exo-1,3-β-glucanase	No	1	4	3
114574	5	Endo-1,4-β-mannanase	Yes	1	3	3
42111	5	Exo-1,3-β-glucanase	No	2	4	4
135369	5	Endo-1,4-β-mannanase	Yes	3	8	6
59826	5	Exo-1,3-β-glucanase	No	9	9	7
110405	5	Endo-1,4-β-mannosidase	Yes	3	7	10
63180	5	Endo-1,4-β-glucanase	Yes	6	14	45
149061	10	Endo-1,4-β-xylanase	Yes	3	0	0
122601	10	Endo-1,4-β-xylanase	Yes	6	1	0
138785	10	Endo-1,4-β-xylanase	Yes	3	7	5
107452	10	Endo-1,4-β-xylanase	Yes	15	36	49
138821	12	Endo-1,4-β-glucanase	Yes	8	18	49
73036	13	Amylase	Yes	9	0	0
61700	15	Amylase	Yes	11	7	7
52752	16	β-Glucan synthesis	No	4	0	0
66072	16	β-Glucan synthesis	No	2	0	0
81698	16	β-Glucan synthesis	No	7	7	2
82316	18	Chitinase	No	2	3	0
122074	18	Chitinase, CBM 5	Yes	0	3	0
118998	18	Chitinase, CBM 5	Yes	3	3	2
45026	18	Chitinase	No	2	3	3
81165	18	Chitinase, CBM 5	Yes	1	5	3
91195	20	β-N-Acetylglucosaminidase	Yes	4	1	0
116582	20	β-N-Acetylglucosaminidase	Yes	2	1	0
81761	27	α-Galactosidase, CBM 35	Yes	1	2	0
117566	27	α-Galactosidase	Yes	11	15	10
104965	28	Endo-polygalacturonase	Yes	8	3	0
110574	28	Polygalacturonase	Yes	2	6	2
54367	28	Exo-polygalacturonase	Yes	0	3	3
117232	28	Exo-rhamnogalacturonase	Yes	1	3	3
6650	28	Exo-polygalacturonase	No	2	6	4
138836	28	Exo-polygalacturonase	Yes	4	6	7
61165	29	α-l-Fucosidase	No	3	5	2
46629	30	Unknown	Yes	3	4	4
119185	31	α-Glucosidase	Yes	10	10	9
141329	31	α-Xylosidase	Yes	10	18	14
81512	35	β-Galactosidase	No	8	13	10
111095	35	β-Galactosidase	Yes	16	24	19
100356	37	Trehalase	No	0	2	3
69366	43	Unknown, CBM 35	Yes	0	3	0
58475	43	Endo-1,5-α-l-arabinase	Yes	3	3	4
107755	47	α-1,2-Mannosidase	Yes	6	6	4
111463	51	α-l-Arabinofuranosidase	Yes	3	6	3
134804	51	α-l-Arabinofuranosidase	Yes	2	9	4
137578	55	Endo-1,3-β-glucosidase	No	5	2	1
126879	55	β-1,3-Glucosidase	Yes	8	12	15
39602	71	Endo-1,3-α-glucosidase	Yes	2	0	0
113553	72	β-1,3-Glycanosyltransferase, CBM 43	Yes	3	4	7
68888	74	Xyloglucanase	Yes	3	10	7
121201	78	Unknown	Yes	2	2	0
136552	78	Unknown	Yes	7	6	4
44058	79	β-Glucuronidase	No	1	6	2
116837	79	β-Glucuronidase	No	3	11	10
120232	88	Glucuronyl hydrolase	Yes	2	2	0
81814	92	Unknown	Yes	7	11	10
108097	95	α-Fucosidase	Yes	6	9	7
121307	115	α-Glucuronidase	Yes	5	0	0
121308	115	α-Glucuronidase	Yes	15	20	14

^aProtein identification (ID) numbers from the Department of Energy (DOE) Joint Genome Institute (JGI) MycoCosm database (28).

^bGlycoside hydrolase families, as defined in the CAZy database (29).

^cPutative functions determined by BLAST searches of Swiss-Prot database (27). CBM, carbohydrate-binding molecule.

^dSecretion signals for detected protein sequences were detected using SignalP algorithm (30).

^eUnique peptide counts for proteins found in wafer sections 0 to 5, 10 to 15, and 20 to 25 mm from the hyphal front.