TABLE 1.
Stage (days) | Input(s)b | Concn of analyte in effluent (mM) |
DNA dayc | |||||
---|---|---|---|---|---|---|---|---|
Sulfate | Sulfide | Nitrate | Nitrite | Acetate | Propionate | |||
I (1–13) | 3 VFA | 0.03 ± 0.08 | 1.63 ± 0.08 | 4.04 ± 0.2 | 0 | |||
II (14–22) | 3 VFA, 4 NO3− | 0.01 ± 0.01 | 2.05 ± 0.02 | 0 | 0 | 8.57 ± 0.11 | 1.06 ± 0.1 | |
III (23–35) | 3 VFA, 8 NO3− | 0.28 ± 0.12 | 1.87 ± 0.08 | 0 | 0 | 6.09 ± 0.13 | 0 | |
IV (36–49) | 3 VFA, 13 NO3− | 1.40 ± 0.08 | 0.67 ± 0.1 | 0 | 0 | 3.01 ± 0.09 | 0 | 45 |
V (50–64) | 3 VFA | 0 | 2.08 ± 0.09 | 9.17 ± 0.42 | 1.9 ± 0.1 | 60 | ||
VI (65–83) | 3 ace, 3 prop, 4 NO3− | 1.05 ± 0.08 | 1.11 ± 0.13 | 0 | 0 | 3.10 ± 0.06 | 0 | 83 |
VII (84–95) | 3 ace, 3 prop, 8 NO3− | 1.67 ± 0.07 | 0.47 ± 0.1 | 0 | 0 | 1.19 ± 0.17 | 0 | 95 |
VIII (96–112) | 3 ace, 3 prop, 13 NO3− | 2.2 ± 0.05 | 0 | 2.4 ± 0.3 | 7.2 ± 0.4 | 0 | 0 | 111 |
IX (113–125) | 3 ace, 3 prop | 0.49 ± 0.06 | 1.72 ± 0.05 | 5.01 ± 0.17 | 0.72 ± 0.09 | |||
X (127–134) | 3 prop | 0 | 2.01 ± 0.03 | 2.48 ± 0.07 | 0.02 ± 0.02 | |||
XI (136–147) | 3 prop, 4 NO3− | 1.70 ± 0.10 | 0.47 ± 0.11 | 0 | 0 | 0.57 ± 0.12 | 145 | |
XII (149–163) | 3 prop, 6 NO3− | 2.21 ± 0.14 | 0.05 ± 0.05 | 0 | 0.04 ± 0.06 | 0 | 0 | 161 |
XIII (164–187) | 3 prop, 2 NO3− | 0.7 ± 0.07 | 1.39 ± 0.07 | 0 | 0 | 1.82 ± 0.05 | 0 |
Data are shown in Fig. 4; the flow rate was 42 ml/day (1.5 PV/day); 2 mM sulfate was injected throughout; butyrate was not detected.
Numbers are concentrations in millimoles ± standard deviations. ace, acetate; prop, propionate.
Data represent the days on which effluent samples were collected for 454 sequencing.