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Activity of lysostaphin, Ami2638A, and CHAPK_CWT-LST against S. aureus in raw milk. (A) Time-kill assay in 33% raw milk (diluted in PBS) spiked with 103 CFU/ml of S. aureus Newbould 305 using 319.5 nM (9 μg/ml) of lysostaphin, 319.5 nM CHAPK_CWT-LST, and 479.5 nM Ami2638A. (B) Time-kill assay as described in panel A but using 100% raw milk instead of diluted milk. (C) Time-kill assay as described for panel A but using 10% raw milk (diluted in PBS).
