TABLE 1.
PGH constructs characterized in this study
| PGH | Backbone plasmid | E. coli strain | Mol mass (kDa) |
|---|---|---|---|
| Lysostaphina | pET-21a | BL21-Gold(DE3) | 28.2 |
| CHAPK_CWT-LSTb | pQE-30 | XL1-Blue MRF′ | 32.2 |
| Ami2638Ab | pQE-30 | XL1-Blue MRF′ | 29.1 |
| CHAPK_CBD2638Ab | pQE-30 | XL1-Blue MRF′ | 35.7 |
| M23LST_CBD2638Ab | pQE-30 | XL1-Blue MRF′ | 30.7 |
| CHAPK_CHAPK_CWT-LSTb | pQE-30 | SURE | 54.3 |
| M23LST_CHAPH5_CWT-LSTa | pET-21a | BL21-Gold(DE3) | 47.8 |
| CHAPH5_LSTa | pET-21a | BL21-Gold(DE3) | 47.6 |
| CHAPTworta | pET-21a | BL21-Gold(DE3) | 17.6 |
a
Construct contains a C-terminal 6×His tag.
b
Construct contains an N-terminal 6×His tag followed by a tobacco etch virus protease cleavage site.