FIG 1.

Effects of culture phases or culture medium components on immunomodulatory properties of OLL2712 cells for murine immune cells. (A and B) BMDCs (A) or PMs (B) were treated with OLL2712 cells (50 μg/ml), prepared at different culture phases (4 h to 16 h) and in medium with (■) or without (□) mono-oleic acid ester Q-17S, for 24 h. (C) BMDCs were treated with OLL2712 cells at indicated concentrations (1 to 50 μg/ml), prepared at different culture phases (8 h or 14 h) and in medium with (+) or without (−) mono-oleic acid ester Q-17S, for 24 h. The temperature and neutralizing pH in OLL2712 cultures were 33°C and pH 5.8. The IL-10 and IL-12 concentrations in the supernatants were measured. The results are presented as means ± SD (n = 4). Data are representative of 2 independent experiments.