FIG 2.

Effects of culture phases or culture medium components on immunomodulatory properties of MEP222801 cells for murine immune cells. BMDCs were treated with MEP222801 cells (50 μg/ml), prepared at different culture phases (5 h or 16 h) and in medium with (+) or without (−) mono-oleic acid ester Q-17S, for 24 h. The temperature and neutralizing pH in MEP222801 cultures were 33°C and pH 5.8. The IL-10 and IL-12 concentrations in the supernatants were measured. The results are presented as means ± SD (n = 4). Data are representative of 2 independent experiments.