FIG 1.

Localization of eYFP fusion proteins. N-terminal fusions of A0674 (CYTH) (A), A3323 (CYTH) (B), A0104 (CHAD) (C), and B1017 (CYTH and CHAD) (D) with eYFP were expressed in R. eutropha during growth on NB medium at 30°C. Cells were stained with DAPI for at least 10 min, immobilized with agarose, and imaged under bright field (left), at a polyP-DAPI-specific wavelength (middle left), and in the green channel (middle right). Merges of all channels are shown in the images on the right. Note the “soluble” eYFP-fusion fluorescence for YFP-A0674 and eYFP-A3323 and the colocalization of eYFP-A0104 and eYFP-B1017 foci with polyP granules. Bars, 2 μm.