FIG 3.

polyP formation and localization of A0104 without fusion to eYFP in R. eutropha harboring pCM62-phaC-A0104. Cells were grown in NB medium at 30°C. The approximate cell shapes are highlighted in the fluorescence images by white dotted lines. Note that all cells form polyP granules. In the exponential growth phase (4 to 8 h; left), only 10% of the cells had cell pole-located polyP granules. In the stationary growth phase (12 to 24 h; right), 90% of the cells had cell pole-located polyP granules. Bars, 2 μm.