TABLE 1.

Numbers and subcellular localization of polyP granules in R. eutropha wild type and in recombinant strains^a

Time (h)	No. of polyP granules per cell (%)
	WT	ΔA0104	eYFP-A0104 (plasmid)	A0104-eYFP (chromosome integrated)
0	1 (>99)	1 (>98)	1 (>99)	1 (>97)
2	1 (90)	1 (90)	1 (70)	1 (80)
	2 (10)	2 (10)	2 (30)	2 (20)
4	1 (80)	1 (80)	1 (50)	1 (25)
	2 (20)	2 (20)	2 (50)	2 (60)
				3 (5)
				4 (10) dividing cells
6	2 (80)	2 (80)	1 (30)	1 (15)
	3 (15)	3 (15)	2 (70)	2 (80)
	4 (5) dividing cells	4 (5) dividing cells	3 (occasionally)	3 (5) dividing cells
8	1 (50)	1 (50)	1 (80)	1 (45)
	2 (50)	2 (50)	2 (20)	2 (50)
				3 (5)
10	1 (80)	1 (80)	1 (80)	1 (80)
	2 (20)	2 (20)	2 (20)	2 (20)
12	1 (90)	1 (90)	1 (>99)	1 (90)
	2 (10)	2 (10)		2 (10)
18	1 (>99)	1 (>98)	1 (>99)	1 (>97)
24	1 (>99)	1 (>98)	1 (>99)	1 (>97)
Localization of polyP at cell poles	Never (<1) (polyP granules between ¼ and ¾ of cell length axis)	Never (<2) (polyP granules between ¼ and ¾ of cell length axis)	Always (>99) (polyP granules never between ¼ and ¾ of cell length axis)	Rarely (≤3) (polyP granules between ¼ and ¾ of cell length axis)

^aCells were grown on NB medium at 30°C. The number of examined of cells varied between 35 (A0104-eYFP chromosome-integrated), 60 (ΔA0104), and 80 (all others). Cells with four polyP granules were always elongated and were undergoing or about to undergo cell division. WT, wild type.