TABLE 2.
List and characteristics of strains and plasmids used in this study
| Strain/plasmid | Relevant characteristica | Source/reference |
|---|---|---|
| Strains | ||
| Escherichia coli JM109 | Cloning strain | DSMZ 3423 |
| E. coli WM3064 | Conjugation strain for M. gryphiswaldense | William Metcalf |
| E. coli S17-1 | Conjugation strain | 44 |
| Ralstonia eutropha H16 | R. eutropha wild-type strain | DSMZ 428 |
| R. eutropha ΔA0104 (SK5693) | Chromosomal deletion of A0104 | This study |
| R. eutropha ΔB1017 (SK5971) | Chromosomal deletion of B1017 | This study |
| R. eutropha ΔA0104+ΔB1017 (SK5972) | Chromosomal deletion of A0104 and B1017 | This study |
| R. eutropha Δppk-all (SK5908) | Chromosomal deletion of seven ppk genes: Δppk1a, Δppk1b, Δppk2a, Δppk2b, Δppk2c, Δppk2d, and Δppk2e | This study |
| R. eutropha A0104-eyfp (SK6364) | Chromosomal integration of eyfp downstream of A0104 | This study |
| R. eutropha Δppk-all A0104-eyfp (SK6365) | Chromosomal integration of eyfp downstream of A0104 in Δppk-all background | This study |
| Magnetospirillum gryphiswaldense MSR-1 | M. gryphiswaldense wild-type strain | DSMZ 6361 |
| M. gryphiswaldense MSR-1 ΔphbCAB | M. gryphiswaldense with deletion of phbCAB | 45 |
| Pseudomonas putida KT2440 | P. putida wild type | DSMZ 6125 |
| Plasmids | ||
| pLO3 | Deletion vector, Tetr, sacB | 46 |
| pLO3-A0104-eyfp | Integration of A0104-eyfp | This study |
| pBBR1MCS2 | Broad host range vector, KANr | 47 |
| pBBR1MCS2-PphaC-eyfp-c1 | Universal vectors for construction of fusions C-terminal to eYFP under the PphaC promoter | 40 |
| pBBR1MCS2-PphaC-eyfp-n1 | Universal vectors for construction of fusions N-terminal to eYFP under the PphaC promoter | 40 |
| pBBR1MCS2-PphaC-eyfp-A0104 | N-terminal fusion of A0104 to eYFP | This study |
| pBBR1MCS2-PphaC-A0104-eyfp | C-terminal fusion of A0104 to eYFP | This study |
| pBBR1MCS2-PphaC-eyfp-B1017 | N-terminal fusion of B1017 to eYFP | This study |
| pBBR1MCS2-PphaC-eyfp-A0674 | N-terminal fusion of A0674 to eYFP | This study |
| pBBR1MCS2-PphaC-eyfp-A3323 | N-terminal fusion of A3323 to eYFP | This study |
| pBBR1MCS2-PphaC-eyfp-PP2307 | N-terminal fusion of PP2307 to eYFP | This study |
| pBAM160-Ptet-MGMSRV2-1987-gfp-c1 | C-terminal fusion of MGMSRV2-1987 to GFP | This study |
a
Resistance against kanamycin (KANr) and tetracycline (Tetr) as indicated.