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MAPK signaling is diminished in Alpl−/− chondrocytes. Cell lysate was isolated from primary rib chondrocytes of Alpl−/− and Alpl+/+ mice after culture with/without inorganic phosphate. MAPK activity was assessed by immunoblotting for phosphorylated and total MAPK protein. Results show diminished basal and phosphate induced levels of phosphorylated MAPK protein in lysate of Alpl−/− cells.
