Figure 7.

CO pretreatment does not rescue MSCs deficient in autophagic proteins from oxidative stress–induced cell death. Passage 3 MSCs were plated in 12-well plates. At 24 hours after plating, cells were pretreated with 250 ppm CO for 16 hours. Cells were then treated with 125 μmol/L H₂O₂ for 24 hours. A control group was left untreated. MTT assay for living cells was performed. MTT was quantified by reading absorbance at 590 nM. Data are shown as MTT absorbance compared with the absorbance of the untreated cells of each group and expressed as a percentage. Data are expressed as mean ± SEM (n = 3–4). *P < 0.001 as compared with its own untreated group; ⁺P < 0.001 as compared with WT H₂O₂; ^#P < 0.001 compared with its own H₂O₂ group without CO.