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. 2017 Mar 21;7:45060. doi: 10.1038/srep45060

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Top: Macroscopic imaging of metastatic burden (bioluminescence imaging, BLI) and nanodrug uptake (fluorescence reflectance imaging, Fl) in excised organs. Bottom: Quantitative analysis of fluorescence intensity in major organs). Metastatic organs were associated with high uptake of the nanodrug. Additional organs of high uptake reflected the natural pathway of metabolism and excretion of the nanoparticles. (b) Histology of tissue sections derived from excised organs. Top: H&E, bottom: fluorescence (Nanodrug: red, Cy5.5; nuclei: blue, DAPI). For lymph nodes and lungs, the whole tissue shown in the image is a metastatic lesion. The bone metastatic lesion is outlined in the image.