Figure 4. Immunostaining showing the expression of E-cadherin and HOXD10 in lung sections from animals with evidence of metastatic regression and animals that failed to regress lung metastases in response to treatment with MN-anti-miR10b and low-dose doxorubicin.

E-cadherin, was highly expressed in the lung metastases of both the regressing and non-regressing groups. The direct miR-10b target, HOXD10, was upregulated in the regressing group compared to the non-regressing group, indicating inefficient inhibition of miR-10b in the animals that failed to regress metastases.