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. 2017 Mar 21;7:44867. doi: 10.1038/srep44867

Figure 4. TCAs inhibit acid ceramidase in human HSCs, leading to accumulation of ceramide.

Figure 4

(A) Diagram of the sphingomyelinase pathway. (B) aSMase enzyme assay results of HSCs treated with ethanol vehicle (Veh) or nortriptyline (Nor, 27 μM) for 48 hours. *p < 0.05. (C) aCDase enzyme assay results of HSCs treated with ethanol vehicle (Veh) or nortriptyline (Nor, 27 μM) for 48 hours and HSCs transfected with nontargeting siRNA (control) or siRNA targeting aCDase mRNA (ASAH1). *p < 0.05. (D and E) Ceramide and sphingosine accumulation was measured in HSCs treated with vehicle or nortriptyline. *p < 0.05.