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. Author manuscript; available in PMC: 2017 Mar 21.
Published in final edited form as: Methods Mol Biol. 2017;1491:11–22. doi: 10.1007/978-1-4939-6439-0_2

Table 1.

Solvent systems utilized for MudPIT analysis

Step 1: 0% Buffer C
Time [min] % Buffer A % Buffer B % Buffer C
0.00 100 0 0
5.00 100 0 0
60.00 55 45 0
70.00 0 100 0
100.00 0 100 0
Step 2: 50% Buffer C
Time [min] % Buffer A % Buffer B % Buffer C
0.00 100 0 0
6.00 100 0 0
6.10 45 5 50
8.00 45 5 50
8.10 95 5 0
15.00 85 15 0
35 00 75 25 0
75.00 45 55 0
80.00 45 55 0
Step 3: 80% Buffer C
Time [min] % Buffer A % Buffer B % Buffer C
0.00 100 0 0
5.00 100 0 0
5.10 15 5 80
8.00 15 5 80
8.10 95 5 0
18.00 85 15 0
63.00 75 25 0
115.00 45 55 0
120.00 45 55 0
Step 4: 100% Buffer C
Time [min] % Buffer A % Buffer B % Buffer C
0.00 100 0 0
4.00 100 0 0
4.10 0 0 100
20.00 0 0 100
20.10 93 7 0
25.00 85 15 0
100.00 70 30 0
184.00 0 100 0
194.00 0 100 0
195.00 100 0 0
200.00 100 0 0
Step 5: 100% Buffer C
Time [min] % Buffer A % Buffer B % Buffer C
0.00 100 0 0
4.00 100 0 0
4.10 0 0 100
14.00 0 0 100
14.10 93 7 0
30.00 70 30 0
50.00 0 100 0
55.00 0 100 0
56.00 100 0 0
60.00 100 0 0