Table 4. IC50 values (μM) for the selected compounds against WT, E305Q, or E578Q VCP/p97 ATPase activities at 200 μM ATP obtained with 8-dose titration.
Compound | WT | D2- Walker B mutant | D1- Walker B mutant |
---|---|---|---|
E578Q | E305Q | ||
DMSO | >30 | >30 | >30 |
DBeQa | 3 | 11.0 | 5.5 |
NMS-873a | 0.038 | 1.3 | 0.038 |
Ebastine | 25 | 16.6 | >30 |
Ivermectin | >30 | >30 | >30 |
Clotrimazol | 12 | 8.2 | 8.0 |
Saquinavir | >30 | >30 | >30 |
Astemizole | 25 | >30 | >30 |
aPositive control.