Figure 2. ΔnpdA exhibited enhanced growth in fatty acids.

WT, ΔnpdA (KO) and the complemented strain (COM) were grown in 7H9-10%OADC-0.05% Tween-80 to mid-log phase (OD₆₀₀ = 0.4–0.6), washed and resuspended in 7H9-0.05% tyloxapol. Aliquots (OD₆₀₀ = 0.5) of the cultures were diluted 1:50 (v/v) into 100 ml 7H9-0.5% BSA-0.085% NaCl-0.05% tyloxapol supplemented with individual carbon sources (5 mM of each): glucose, glycerol, sodium acetate, sodium propionate or oleic acids. The cultures were grown at 37 °C for 24 days. Results are combined data (mean ± s.d.) from two independent experiments, and each experiment was done in triplicate. Statistically significant differences were found between ΔnpdA and WT or between ΔnpdA and the complemented strain when grown in oleic acids or propionate (A and B) as analyzed by two-way ANOVA (p < 0.05).