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. 2017 Mar 21;7:44817. doi: 10.1038/srep44817

Figure 5. Electrical stimulation of axons of olfactory receptor neurons evokes Naspm-sensitive Ca2+ transients in GCaMP6s-expressing astrocytes.

Figure 5

(a) Ca2+ transients evoked by electrical stimulation in the absence of receptor blockers, in the presence of a mix of receptor blockers (2 μM MPEP, antagonist of mGluR5; 30 μM MRS2179, P2Y1 receptor antagonist; 100 μM D-APV, NMDA receptor antagonist; 1 μM SCH442416, type 1 dopamine receptor antagonist; 100 μM CPCCOET, mGluR1 antagonist; 2 μM SCH23390, A2A receptor antagonist) and after addition of Naspm (50 μM). Inset: First Ca2+ response (control) at larger time scale. Inset scale bars: 20 s, 200% ΔF/F0. (b) Normalized averaged amplitudes of Ca2+ rises evoked by electrical stimulation.