FIG. 2.

Quantitation of human antigen R (HuR) cytoplasmic translocation in injured spinal cord. Immunohistochemistry sections were analyzed for merged (yellow) signal of HuR and glial fibrillary acidic protein immunoreactivity using ImageJ software with a colocalization macro (see Methods). Representative fields where loci meeting the threshold for merged signal are shown in the upper panel. The graph shows the mean ± standard error of the mean of four independent sections from a mouse undergoing T10 contusion or sham injury, with two random fields counted per section. **p < 0.0001. Color image is available online at www.liebertpub.com/neu