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(A) Structures of the dimer 15c and the gem-dimethyl derivative 15d. (B) DNA alkylation assay employing linearized pBR322 DNA and the dimer 15c or the gem-dimethyl derivative 15d. Conditions: Linearized pBR322 DNA (20 μM in base pairs), 15c (10 μM) or 15d (500 or 100 μM), 37 °C, 0.1–15 h.