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. 2017 Mar 21;16:50. doi: 10.1186/s12934-017-0662-4

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Microscopy analysis of IB formation. a E. coli TOP10F’ cells carrying an empty vector (EV) and cells expressing indicated proteins were analyzed by phase-contrast microscopy. Examples of IBs are indicated with a white arrow; examples of IBs seemingly formed at the new-pole of interconnected cells are indicated with a black arrow; examples of clustered IBs are indicated with a black arrowhead. Scale bar 2 μm. b Electron microscopy analysis of IBs isolated from TOP10F’ cells overexpressing indicated proteins. Representative IBs of average size are shown. Scale bar 500 nm