Table 1.
The characteristics of primers used for real-time RT-PCR assays
| Accession number | Target gene | Primer sequence | Product size (bp) |
|---|---|---|---|
| NM_001101.3 | β-actin | Forward: 5′ –TCAGAGCAAGAGAGGCATCC- 3′ Reverse: 5′ –GGTCATCTTCTCACGGTTGG- 3′ |
187 |
| NM_001004311.3 | Figla | Forward: 5′ –TCGTCCACTGAAAACCTCCAG- 3′ Reverse: 5′ –TTCTTATCCGCTCACGCTCC- 3′ |
76 |
| NM_000899.4 | Kit ligand | Forward: 5′ –AATCCTCTCGTCAAAACTGAAGG- 3′ Reverse: 5′ –CCATCTCGCTTATCCAACACTGA- 3′ |
163 |
| NM_001288828.2 | Gdf9 | Forward: 5′ – GAAGTGGGCTGACTGGGTG - 3′ Reverse: 5′ – TGCAAAGCTCTGGAGTCTGG - 3′ |
166 |
| NM_181446.2 | Fshr | Forward: 5′ –CTGGCAGAAGAGAATGAGTCC- 3′ Reverse: 5′ –TGAGGATGTTGTACCCGATGATA- 3′ |
157 |