Table 1.

Primers and protocols used for PCR amplification of GDF9 and BMP15

Gene	Location	Primer sequence	PCR amplification protocol
GDF9
	Exon 1	F,5′-TAGTCCACCCACACACCTGA-3′; R,5′-CCAGAAGCCTGAGAACCA -3′ F,5′-TTCCTCCTTTGGTTTTGCTG-3′; R,5′-AAAGCTCTGGAGTCTGGCTG -3′ F,5′-TTCTATCTGTTGGGCGAGGT-3′; R,5′-CATCTTCCCTCCACCCAGT-3′ F, 5′-CTGCCTGTTGTGTTGACTGA-3′; R,5′-TCTGAATCCATTTGTGTTTCTTTC-3′	94°C for 5 min, followed by 30 cycles at 94°C for 30 s, 58°C for 30 s and 72°C for 30 s, then finally 72°C for 7 min
	Exon 2	F,5′-CTCTCGGCAGAGCTCCATAC-3′; R,5′-GGGGACACCAGAGTCATGTT-3′ F,5′-CGCAGAGGTCAGGAAACTGT-3′; R,5′-GGTCTTGGCACTGAGGAGTC-3′ F,5′-TGAAAGACCAGCTGGAGCA-3′; R,5′-TCAGATTGAAGGAAGCTGGG-3′ F,5′-TCGGTATGGCTCTCCAGTTC-3′; R,5′-AATATATCAAGCTTTCTCTTGAAG-3′	94°C for 5 min, followed by 30 cycles at 94°C for 30 s, 58°C for 30 s and 72°C for 30 s, then finally 72°C for 7 min
BMP15
	Exon 1	F, 5′-TTGTGTTGGGGCCTGTTGTT-3′; R,5′-GGTACAACTCCAGCATGTACC-3′ F,5′-GCTGCTAGAAGAATCCCCTG-3′; R,5′-AACCCACCAATTCCCTTTT-3′	94°C for 5 min, followed by 30 cycles at 94°C for 60 s, 58°C for 60 s and 72°C for 60 s, then finally 72°C for 7 min
	Exon 2	F,5′-AATATTCATGTTAAGAGGTAAGA-3′; R,5′-AGGAAGGGAAGTGGTTGGTT-3′ F,5′-TCAATCTCTCCTGCCATGTGG-3′; R,5′-TGTCCAAGGATGAAGAGCC -3′ F,5′-TGGTCTTGAGCTCTGGCATG-3′; R,5′-CTGATTTGGAAAGGGTGGAG-3′	94°C for 5 min, followed by 30 cycles at 94°C for 30 s, 58°C for 30 s and 72°C for 30 s, then finally 72°C for 7 min