Table 1.

Fermentation properties for engineered and evolved S. cerevisiae strains

Medium	Aerobic YPDX			Aerobic ACSH
Strain	SCY62+EaDAcT	H1246+EaDAcT	4KO+EaDAcT	SCY62+EaDAcT	H1246+EaDAcT	4KO+EaDAcT
Acetyl-TAG titera	2400 ± 65	1500 ± 180	3000 ± 42	2200 ± 95	160 ± 19	1100 ± 6.2
Volumetric acetyl-TAG titerb	15 ± 2.0	6.9 ± 0.11	32 ± 2.5	14 ± 0.13	0.30 ± 0.036	7.9 ± 0.087
lcTAG titerc	6800 ± 0.20	ND	4200 ± 44	6300 ± 0.10	96 ± 1.1	2300 ± 2.2
Volumetric lcTAG titerd	43 ± 5.9	ND	45 ± 3.3	4.0 ± 0.37	0.18 ± 0.016	16 ± 0.10
% acetyl-TAGse	31 ± 0.21	100 ± 0	48 ± 0.52	31 ± 0.097	67 ± 0.99	38 ± 0.36
Estimated growth ratef	0.34 ± 0.049	0.25 ± 0.035	0.58 ± 0.044	0.021 ± 0.0004	0.074 ± 0.002	0.29 ± 0.0003
YgacTAG/glc	260 ± 9.5	130 ± 9.8	510 ± 48	120 ± 18	8.0 ± 1.5	120 ± 0.67
YhlcTAG/glc	750 ± 32	ND	710 ± 65	330 ± 51	4.8 ± 0.76	250 ± 2.6

ND not detected

^aIn μg of acetyl-TAG/g of dry cell weight (DCW)

^bIn mg of acetyl-TAG/L

^cIn μg of lcTAG/g of DCW

^dIn mg of lcTAG/L

^eMole percentage of acetyl-TAG produced out of the total TAG produced

^fIn g of DCW/L/h within 18 h (in YPDX medium) or 24 h (in ACSH medium) of growth

^gYield in μg of acetyl-TAG produced/g of glucose consumed

^hYield in μg lcTAG produced/g of glucose consumed