Fig. 3.

Chrysin induced HCC cell apoptosis by reducing HK-2 in mitochondria. a, HCC cells were treated with chrysin for 24 h, the mitochondria fractions was extracted and examined by western blotting to detect the change of indicated protein. b, HCC-LM3 cell was treated with chrysin for 24 h and the lysates of mitochondria fractions were immunoprecipitated with HK-2 or VDAC-1 antibodies, then the binding affinity was analyzed with western blotting analysis. c, HCC cells were treated with various chrysin for 24 h and cell lysates were probed with indicated antibodies