Fig. 1.

MK2206 does not lead to temozolomide/radiation sensitization in glioma monolayer cultures. a U87MG cells were treated for 72 h with MK2206 at indicated concentrations and treated with 5 μM TMZ or 4 Gy irradiation or the combination of both. The combination index of the triple combination was calculated at each time point. A CI < 0.8 indicates synergy, 0.8 < > 1.2 additive, > 1.2 antagonism. Data points represent means, ±SD (n = 3). b U251 cells were treated with 1uM MK2206 and or 4 Gy irradiation in different schedules. Top: MK was given 24 h before (Pre-RT) irradiation and plated for colony formation; Middle: cells were treated with MK and immediately irradiated (Post-RT), cells were plated 24 h later for colony formation; Bottom: cells were treated with MK 24 h before irradiation and cells were plated for colony formation in the presence of 1 μM MK2206. Columns represent means, ±SD (n = 2). c U87MG cells were treated with 1 μM MK and/or with 4 Gy irradiation and lysed at indicated time points