Fig. 5.

OX40/OX40L blockade in vitro. a Cell proliferation in the CII- or CD3-stimulated splenocytes of CIA mice in the presence of anti-OX40L mAb (2.5 μg/ml, n = 19; 5.0 μg/ml, n = 19; 10.0 μg/ml, n = 19) or IgG controls (n = 19). b, c Cytokine secretion in the supernatants of CII-stimulated (b) or CD3-stimulated (c) splenocytes from CIA mice in the presence of anti-OX40L mAb (5.0 μg/ml, n = 8) or in IgG controls (n = 8). Bars show means ± SD; * P < 0.05, ** P < 0.01, *** P < 0.001. CIA Collagen-induced arthritis, CII Collagen type II, IgG Immunoglobulin G, mAb Monoclonal antibody