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. 2017 Mar 21;16:66. doi: 10.1186/s12943-017-0619-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — VASH2 decreases the sensitivity of pancreatic cancer cells to gemcitabine in vitro. After treatment with 25 or 50 nM gemcitabine for 48 h, the apoptosis rate of PANC-1-EGFP, PANC-1-VASH2, SW1990-scramble, and SW1990-shVASH2 was indicated by flow cytometric determination of Annexin V and 7-AAD staining. Upper-right + lower-right quadrants indicate apoptotic cells. Apoptosis is quantified in the bar graphs for PANC-1-EGFP and PANC-1-VASH2 (top) and SW1990-scramble and SW1990-shVASH2 (bottom). *P < 0.05