Fig. 5.

JUN is upregulated by VASH2, binds to the RRM2 promoter, and increases transactivation of RRM2. a and b qPCR analysis and immunobloting for JUN in PANC-1-VASH2, PANC-1-EGFP, SW1990-shVASH2, and SW1990-scramble cells. c JUN and RRM2 immunobloting in PANC-1-EGFP and PANC-1-VASH2 transfected with siRNA against JUN. d diagram of three predicted JUN-binding sites within RRM2 promoter (-2147/+1) by SABiosciences and Jaspar online software. e ChIP (JUN) analysis for three predicted JUN-binding sites of RRM2 promoter in PANC-1-EGFP, PANC-1-VASH2, SW1990-scramble and SW1990-shVASH2 cells. f and g dual luciferase reporter assays for PGL3-WT/PGL3-MUT reporter activity co-transfected with or without a JUN expression plasmid in PANC-1, PANC-1-VASH2, SW1990-scramble, and SW1990-shVASH2 cells. The relative promoter activity was calculated as firefly luminescence/Renilla luminescence. *P < 0.05