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. 2017 Mar 15;51:75–88. doi: 10.1016/j.actbio.2017.01.028

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© 2017 Elsevier Ltd. All rights reserved.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 4 — Degradation profile of acellular Scl2 hydrogels. (A) Degradation of hydrogels cross-linked via the MMP7-cleavable peptide and exposed to 30 ng/mL recombinant human MMP1, 2, 7, or 13 over time characterized as dry weight loss and expressed as a percentage of initial dry weight. (B) Degradation of hydrogels cross-linked via the MMP7-cleavable or ScrMMP7 peptides and exposed to 30 ng/mL recombinant human MMP7 over time characterized as dry weight loss and expressed as a percentage of initial dry weight. (C) Release of cystamine-FITC from hydrogels cross-linked via the MMP7-cleavable or ScrMMP7 peptides and exposed to 0.2 μg/mL recombinant human MMP7 over time characterized as fluorescent signal output. Trypsin-driven degradation is used as a positive control. Chondrogenic medium without exogenous enzymes is used as a negative control. Values represent means ± SD. ^**p < 0.01 versus ScrMMP7-HHACS-Scl2 (n = 3).